LLilianingene.hashnode.dev·Jan 9 · 2 min readThe Role of Stable Monoclonal Cell Lines in Reproducible ResearchStable cell lines constitute foundational tools in modern life science research, particularly for studies requiring sustained gene expression or long-term gene silencing across extended experimental timelines. In contrast to transient transfection sy...00
LLilianingene.hashnode.dev·Nov 13, 2025 · 3 min readInsights from Ubigene: CRISPR Library Screening Reveals Genetic Mechanisms of Mouse Tumor MetastasisTumor metastasis is the leading cause of cancer-related deaths and represents one of the most complex processes in oncology. It involves a multistep cascade including epithelial–mesenchymal transition (EMT), invasion, intravasation, circulation, extr...00
LLilianingene.hashnode.dev·Jul 18, 2025 · 4 min readFrom Concept to Cell Line: A Practical Guide to CRISPR Knockout Cell Line GenerationThe CRISPR-Cas9 genome editing system has transformed the landscape of molecular biology, enabling researchers to disrupt gene function with unparalleled precision. One of the most common applications of this technology is the creation of knockout (K...00