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First you must get the file from the request. Use multer as middleware.

const multer = require('multer')

const uploader = multer()
const withResumeFile = uploader.single('resume')

app.post('/apply', withResumeFile, (req, res) => {
  const resume = req.file
  const mailOptions = {
    filename: resume.originalname,
    contentType: resume.mimetype,
    encoding: resume.encoding,
    attachments: [{ content: resume.buffer }],
  }
})

So you can get the actual file in the request.